Malaysian Agriculture Repository >
Institute >
Publications >

Please use this identifier to cite or link to this item: http://agris.upm.edu.my:8080/dspace/handle/0/16142

Title: Protease purification from Bacillus amyloliquefaciens B7 using aqueous two-phase system (ATPS)
Authors: Nadiah Syuhada Abd Samad
Azura Amid
Dzun Noraini Jimat
Nurul Aqilah Ab. Shukor
Issue Date: 2017
Citation: International Food Research Journal (Malaysia), 24 (suppl.), p. S292-S297
Abstract: Bacillus amyloliquefaciens B7 was isolated from the fermented fish sauce and identified as protease producer. Generally, in downstream processing, purification of enzymes consumes higher cost regarding reagents and equipment used. Moreover, harsh purification methods used might cause denaturation of the enzymes. Therefore, there is a high demand for efficient and low cost extraction and purification methods. Aqueous two-phase system (ATPS) is an alternative method that should be considered as it is simple, rapid separation yet cause little denaturation. Protease produced by B. amyloliquefaciens B7 was partitioned using two different ATPS, which were PEG/potassium phosphate and PEG/sodium citrate. Results showed the highest enzyme activity was found in interface phase with the ATPS system of 27% (w/w) PEG1500/34% (w/w) sodium citrate. Later, the ATPS conditions (pH, temperature, the concentration of selected salt and PEG) were optimized by using response surface methodology. The optimum conditions for ATPS purification were observed in ATPS conditions at pH 7 and 35°C with the enzyme activity of 0.20± 0.01 U/ml.
License: http://www.oceandocs.org/license
URI: http://agris.upm.edu.my:8080/dspace/handle/0/16142
Related document: http://www.ifrj.upm.edu.my/24%20(07)%202017%20supp...
ISSN: 2231-7546
Appears in Collections:Publications

Files in This Item:

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


© 2013 Developed by Bahagian Sistem & Teknologi Maklumat. Perpustakaan Sultan Abdul Samad. Universiti Putra Malaysia.