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|Title: ||Transfer of insect resistance gene into oil palm|
|Authors: ||Muhammad Rashdan Muad|
Leaw, Chui Li
Yeun, Li Buey
Yap, Winnie Soo Ping,
|Issue Date: ||1999|
|Publisher: ||Malaysian Plant Protection Society|
|Citation: ||Tropical Plant Protection in the Information Age : Proceedings Plant Protection in the Tropics: Kuala Lumpur, Malaysia, 15-18 Mac 1999, p. 419-421|
|Abstract: ||Immature embryos of the oil palm (Elaeis guineensis Jacq.), variety tenera, were transfonned with a trypsin inhibitor gene from cowpea using microprojectile bombardment. Presence of the transgene was detennined using Polymerase Chain Reaction (PCR) and Southern Blot analyses. Using primers specific to the CpTi gene, the expected 314 bp fragment was amplified from 87% of randomly selected plants over a period of 12 months. The presence of the CpTi gene was further continned by PCR-Southern hybridization. Further molecular analyses are now in progress to detennine the integration of the genes into the genome and its stability in its new environment.|
|Appears in Collections:||Publications|
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