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|Title: ||Genetic transformation as a system to introduce foreign genes into plants|
|Authors: ||Ahmad Parveez Ghulam Kadir|
|Issue Date: ||2006|
|Publisher: ||Malaysian Palm Oil Board (MPOB)|
|Citation: ||Proceedings of the International Symposium on Oil Palm Genetic Resources and their Utilization: Kuala Lumpur, (Malaysia), 8-10 Jun 2000, p. 585-619|
|Abstract: ||Genetic transformation is a process where by foreign DNA is stably and functionally introduced into living cells. The transformed cells can then be selected and regenerated into whole transgenic plants. Various methods are available for plant gene transfer and among them are Agrobacterium-mediated, protoplast-mediated (via polyethylene glycol and electroporation), biolistics, microinjection, ultrasonication, tissue electroporation, microlaser and liposome-mediated techniques. Among the various methods available, Agrobacterium mediated, protoplast-mediated and biolistics are the most reliable and repeatable. Agrobacterium-mediated gene transfer is the pioneer method, However, biolistics has become a popular transformation method, not only because it is simple, but also it has no species, cell type or tissue limitation. Most interestingly, biolistics can transform most of the agronomically important crops. Since the production of the first transgenic tobacco plant in 1983, the number of transgenic plant species has increased drastically. The type of gene transferred has changed from marker and selectable genes to more economically important genes. The products of transgenic plants have improved from antibiotic and herbicide resistance to disease/pest resistance and agronomic traits. In this paper, descriptions of some of the transformation methods and examples of various products of transgenic plants will be
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