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|Title: ||The genetic diversity of the American oil palm, Elaeis oleifera (kunth), Cortes revealed by nuclear RFLP markers|
|Authors: ||Barcelos, E.|
|Issue Date: ||2006|
|Publisher: ||Malaysian Palm Oil Board (MPOB)|
|Citation: ||Proceedings of the International Symposium on Oil Palm Genetic Resources and their Utilization: Kuala Lumpur, (Malaysia), 8-10 Jun 2000, p. 544-555|
|Abstract: ||The Restriction Fragment Length Polymorphism (RFLP) technique was used to assess the genetic diversity, its organization and genetic relationships among 36 American oil palm [Elaeis oleifera (Kunth), Cortes] populations from Brazil, Peru, French Guyana, Surinam, north Colombia and Central America, covering a large part of the natural distribution of the species. Polymorphism in 241 accessions was revealed with 37 nuclear cDNA probes and submitted to Factorial Analysis of Correspondences (FA C), cluster analysis and population genetics analysis (Fstatistics). The genetic diversity of E. oleifera is strongly structured by geographical origin, with four groups clearly distinguished: Brazil, Surinam/French Guyana. North Colombia/Central America and Peru. Within the Amazon basin, there is a moderate structure that corresponds to the major tributaries of the Amazon river. From the 37 polymorphic RFLP probe/enzyme combinations used, 19 probes (51%) presented simple restriction profiles with one or two bands per plant, suggesting a single locus with different alleles, allowing allelic co-dominant coding for them. This led to the identification of 59 alleles for all 19 loci. Three rare alleles. with frequencies lower than 5% were present in the Brazillian populations, while no other allele was found to be rare or very frequent (>95%) in the whole species, nor in any ofthe sub-geographic groups. Very low levels of polymorphism and heterozygosity were observed in the non-Brazilian groups (Surinam/French Guyana, north Colombia/Central America and Peru) with some ofthese being even monomorphic and completely homozygous for the studied RFLP locus. A high genetic diversity was detected in E. oleifera by RFLP nuclear analysis, with a mean of3.1 alleles in the 19 loci and H Nei =0.404. These values are higher than the genetic diversity estimated for the African E. guineensis by using the same probes over 51 accessions covering a broad area of the distribution of this species, and are higher also than in other perennial species studied elsewhere.|
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