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|Title: ||Micropropagation of Orthosiphon stamineus|
|Authors: ||Chan L. K.|
Lee, W. L.
|Issue Date: ||2000|
|Publisher: ||Forest Research Institute Malaysia (FRIM)|
|Citation: ||Proceedings of the Seminar on Medicinal and Aromatic Plants: Towards Bridging Science and Herbal Industry: Kuala Lumpur (Malaysia), 12-13 Sep 2000, p. 170-175|
|Abstract: ||A micropropagation method for Orthosiphon stamineus, a traditional medicinal plant, has been developed. Nodal segments from in vitro growing shoots were used as explants. Explants were cultured on Murashige and Skoog (MS) medium supplemented with various combination of benzyladenine (BA) and indole-3-butyric acid (IBA), 30 g/l sucrose and 7 g/l agar. The highest shoot poliferation rates were obtained on MS supplemented with 1.5 mg/l BA with an average of sixteen (15.8) shoots per explant over a period of six weeks. Rooting occurred when in vitro shoots were cultured in basic MS medium or with the addition of 6 mg/l of IBA. An average of six roots (5.6) per shoot were produced when cultured in MS supplemented with 6 mg/l IBA for two weeks. All the in vitro plantlets survived when transferred to the external environment.|
|Appears in Collections:||Publications|
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